17 Georgian Bay Forever Phragmites Report 2021 DNA Sampling Along the North River and Coldwater River there were many sites of uncertainty found. The Phragmites in these locations were large, dense and tall like invasive Phragmites but also displayed a lighter green/yellow colour, red stalks and sparse seed heads similar to the native strain. In discussions with SSEA and MTM Conservation Association, there was still uncertainty in the identification of these sites. In the fall of 2021, GBF’s Project Coordinator collected 7 samples and sent them to the Wendell Lab at Oakland University for analysis (https://doi.org/10.1016/j.jglr.2021.08.002). 6 out of 7 of these samples were identified as the native lineage, Phragmites australis americanus. With these results, we are able to better plan for the 2022 season, knowing which sites to cut and which to leave. Our results have led us to determined that Matchedash Bay is home to a total of 28 native stands of Phragmites in addition to the 44 invasive stands. Community Cuts In August, GBF worked with MTM Conservation Association to cut 3 sites found in the Beaver Pond, also known as Heron Pond, accessible only by foot off of Quarry Road. Though these sites were not overly dense, the stalks found growing here were some of the tallest we have seen across the eastern shoreline of Georgian Bay. After cutting these sites we found 2 others located on the other side of the pond that we aim to cut in 2022. Figure 16: Nicole taking samples for DNA testing on the North River. Results showed this as native Phragmites. Figure 17: GBF and MTM staff and volunteers proud of their long, hard day Phragbusting at Heron Pond!