GBF 2019 Phrag Report

27 DNA Analysis – investigating if there is hybrid species in Georgian Bay There have been haplotypes of hybrids, of the invasive and native found in a small number of areas in the United Sates (Las Vegas is one). In recent years we have seen native Phragmites stands display some similar qualities to invasive stands - dense, and tall for example. GBF wanted to investigate. Georgian Bay Forever sent 5 samples to Doug Wendell at Oakland University in the fall of 2019 to measure the Chloroplast Loci in native and invasive Phragmites . All 5 stands tested were all North of Parry Sound, 4 samples sent came back as native and 1 being invasive. The native stands sampled were all tall, dense stands, but had the red stock. They did not test as “invasive”. That is not the end of the story though. At this time, there is no test to measure specifically against a hybrid type – it is not profiled. We can only identify in these 2 buckets (native and invasive). GBF knows a university (Trent) is working on this issue, and we also know that Oakland University lab is working on a hybrid test. We will continue to follow-up with both and continue to test samples, especially where native and invasive are in close proximity or even mixed together. The locations of 5 phragmites samples sent to Wendall Lab for genetic testing. The left graphic is of the resultant gel showing the DNA polymorphisms between invasive and native Phragmites. The invasive DNA is not cut by the RsaI digest, but is cut by the HhaI digest, whereas the native is cut by the RsaI digest, and not cut by the HhaI digest Source: https://www.greatlakesphragmites.net/phragbasics/native-vs- nonnative/

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